Ion exchange chromatography media, DEAE Sephacel™

of the protozoan Trichomonas vaginalis Donne The four step purification included ion-exchange chromatography (DEAE-Sephacel and Q-Sepha

Glucosidase I was purified about 1900-fold from yeast microsomal preparations by DEAE-Sephacel chro- matography, affinity chromatography on

deae sephadex Chromatography on DEAE-Sephacel The active fractions of the preceding step were pooled and dialyzed overnight against M potassium phos- phate

deae ceramic hyperd The supernatant then was clarified by centrifugation at 105,000 x g,, for 60 min and loaded onto a x 40-cm column of DEAE-Sephacel at 24 mlh